Confirm with me in advance on the protocol that you decide to choose1. The protocol should not be more than five pages long. It can be single-spaced or double-spaced. The content of the protocol is the main factor in the grade.2. Include the following in your protocol:o Titleo Introduction: an introduction with the experimental purpose of the protocol and the theory involved.o Materials and Methods: any equipment and reagents needed (include concentration and pH). Separate the equipement from the reagents and use two columns to save space if you need to.i. Ex: 2-20 ml Micropipettorii. Ex: TE buffer pH 8 (10 mM Tris, 1 mM EDTA)o Methods: type out the bulleted methods section in detail. Include any equipment and reagents that are used for each step, and the volume for the reagents that is required. i. Include any possible controls that can be used. This includes any positive or negative controls.CLICK HERE FOR MORE INFORMATION ON THIS PAPER? PRICE?..1. Ex: For SDS-PAGE use BSA and a well with out the protein sampleii. List the purpose of each reagent and the technique used in your methods! This is a major part of the protocol and a large part of your grade on this paper! o Safety: Any safety notices for the reagents or the equipmento References: don?t forget these. 5 reference minimum.Example protocols: ú DNA isolation: not using a kit. You must list the reagents that are needed (MD5 is not an appropriate reagent for example).ú PCRú SDS PAGE of an unknown sampleú DNA agarose electrophoresisú Passaging (trypsin or scraping) and cryopreservation of a eukaryotic cell lineú Another protocol that you are either interested in due to prior or future work. If you choose to do this, confirm with me.Due date is Friday, the 13th of December. This is the final day of finals week. I pushed the date back to give more flexibility for your finals, as I don?t want you to stress any more than needed. I would highly recommend that you start this prior to finals so you can focus on your exams. Please contact me with any questions or concerns with the protocol; however, please do not wait until the last minute to start or to ask me. Good luck on finals!CLICK HERE FOR MORE INFORMATION ON THIS PAPER? PRICE?..
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