) Compound P binds with X to form the complex PX. A series of solutions was prepared with the total P concentration remaining fixed at 1.00 x10-5 M in all solutions. Both P and X do not absorb at 437 nm, but PX has an absorption maximum at 437 nm. The following table shows how the absorbance at 437 nm in a 5.00 cm cell depends of [X], using the assumption that [X]is approximately equal to XT because XT >>*P+. Make a Scatchard plot of ?A/*X+ versus ?A in order to find the equilibrium constant K for this binding event.
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